Fluorescence two-dimensional difference gel electrophoresis for biomaterial applications

Laura E. McNamara, Matthew J. Dalby, Mathis O. Riehle, Richard Burchmore

Abstract

Fluorescence two-dimensional difference gel electrophoresis (DiGE) is rapidly becoming established as a powerful technique for the characterization of differences in protein expression levels between two or more conditions. In this review, we consider the application of DiGE—both minimal and saturation labelling—to biomaterials research, considering the challenges and rewards of this approach.

Footnotes

  • One contribution to a Theme Supplement ‘NanoBioInterface: crossing borders’.

  • Received May 8, 2009.
  • Accepted June 4, 2009.
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