Skip to main content
  • Other Publications
    • Philosophical Transactions B
    • Proceedings B
    • Biology Letters
    • Open Biology
    • Philosophical Transactions A
    • Proceedings A
    • Royal Society Open Science
    • Interface
    • Interface Focus
    • Notes and Records
    • Biographical Memoirs

Advanced

  • Home
  • Content
    • Latest issue
    • All content
    • Subject collections
    • Videos
  • Information for
    • Authors
    • Reviewers
    • Readers
    • Institutions
  • About us
    • About the journal
    • Editorial board
    • Author benefits
    • Policies
    • Citation metrics
    • Publication times
    • Open access
  • Sign up
    • Subscribe
    • eTOC alerts
    • Keyword alerts
    • RSS feeds
    • Newsletters
    • Request a free trial
  • Submit
You have accessRestricted access

A biophysical approach to daunorubicin interaction with model membranes: relevance for the drug's biological activity

Ana Catarina Alves, Daniela Ribeiro, Miguel Horta, José L. F. C. Lima, Cláudia Nunes, Salette Reis
Published 30 August 2017.DOI: 10.1098/rsif.2017.0408
Ana Catarina Alves
UCIBIO, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira no. 228, 4050-313 Porto, Portugal
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Ana Catarina Alves
Daniela Ribeiro
UCIBIO, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira no. 228, 4050-313 Porto, Portugal
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Miguel Horta
UCIBIO, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira no. 228, 4050-313 Porto, Portugal
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
José L. F. C. Lima
UCIBIO, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira no. 228, 4050-313 Porto, Portugal
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Cláudia Nunes
UCIBIO, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira no. 228, 4050-313 Porto, Portugal
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Cláudia Nunes
  • For correspondence: cdnunes@ff.up.pt
Salette Reis
UCIBIO, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira no. 228, 4050-313 Porto, Portugal
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Salette Reis
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

Daunorubicin is extensively used in chemotherapy for diverse types of cancer. Over the years, evidence has suggested that the mechanisms by which daunorubicin causes cytotoxic effects are also associated with interactions at the membrane level. The aim of the present work was to study the interplay between daunorubicin and mimetic membrane models composed of different ratios of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), sphingomyelin (SM) and cholesterol (Chol). Several biophysical parameters were assessed using liposomes as mimetic model membranes. Thereby, the ability of daunorubicin to partition into lipid bilayers, its apparent location within the membrane and its effect on membrane fluidity were investigated. The results showed that daunorubicin has higher affinity for lipid bilayers composed of DMPC, followed by DMPC : SM, DMPC : Chol and lastly by DMPC : SM : Chol. The addition of SM or Chol into DMPC membranes not only increases the complexity of the model membrane but also decreases its fluidity, which, in turn, reduces the amount of anticancer drug that can partition into these mimetic models. Fluorescence quenching studies suggest a broad distribution of the drug across the bilayer thickness, with a preferential location in the phospholipid tails. The gathered data support that daunorubicin permeates all types of membranes to different degrees, interacts with phospholipids through electrostatic and hydrophobic bonds and causes alterations in the biophysical properties of the bilayers, namely in membrane fluidity. In fact, a decrease in membrane fluidity can be observed in the acyl region of the phospholipids. Ultimately, such outcomes can be correlated with daunorubicin's biological action, where membrane structure and lipid composition have an important role. In fact, the results indicate that the intercalation of daunorubicin between the phospholipids can also take place in rigid domains, such as rafts that are known to be involved in different receptor processes, which are important for cellular function.

Footnotes

  • Electronic supplementary material is available online at https://dx.doi.org/10.6084/m9.figshare.c.3858292.

  • Received June 1, 2017.
  • Accepted August 4, 2017.
  • © 2017 The Author(s)
http://royalsocietypublishing.org/licence

Published by the Royal Society. All rights reserved.

View Full Text

Sign in for Fellows of the Royal Society

Fellows: please access the online journals via the Fellows’ Room

Not a subscriber? Request a free trial

Log in using your username and password

Enter your Journal of The Royal Society Interface username.
Enter the password that accompanies your username.
Forgot your user name or password?

Log in through your institution

You may be able to gain access using your login credentials for your institution. Contact your library if you do not have a username and password.

Pay Per Article - You may access this article or this issue (from the computer you are currently using) for 30 days.

Regain Access - You can regain access to a recent Pay per Article or Pay per Issue purchase if your access period has not yet expired.

PreviousNext
Back to top
PreviousNext
August 2017
Volume 14
, issue 133
Journal of The Royal Society Interface: 14 (133)
  • Table of Contents
  • About the Cover
  • Index by author
  • Ed Board (PDF)

Keywords

drug–membrane interactions
daunorubicin
liposomes
partition
location
fluidity
Share
A biophysical approach to daunorubicin interaction with model membranes: relevance for the drug's biological activity
Ana Catarina Alves, Daniela Ribeiro, Miguel Horta, José L. F. C. Lima, Cláudia Nunes, Salette Reis
J. R. Soc. Interface 2017 14 20170408; DOI: 10.1098/rsif.2017.0408. Published 30 August 2017
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Connotea logo Facebook logo Google logo Mendeley logo
Email

Thank you for your interest in spreading the word on Journal of The Royal Society Interface.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
A biophysical approach to daunorubicin interaction with model membranes: relevance for the drug's biological activity
(Your Name) has sent you a message from Journal of The Royal Society Interface
(Your Name) thought you would like to see the Journal of The Royal Society Interface web site.
Print
Manage alerts

Please log in to add an alert for this article.

Sign In to Email Alerts with your Email Address
Citation tools
Research article:

A biophysical approach to daunorubicin interaction with model membranes: relevance for the drug's biological activity

Ana Catarina Alves, Daniela Ribeiro, Miguel Horta, José L. F. C. Lima, Cláudia Nunes, Salette Reis
J. R. Soc. Interface 2017 14 20170408; DOI: 10.1098/rsif.2017.0408. Published 30 August 2017

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Article reuse

  • Article
    • Abstract
    • 1. Introduction
    • 2. Material and methods
    • 3. Results and discussion
    • 4. Conclusion
    • Data accessibility
    • Authors' contributions
    • Competing interests
    • Funding
    • Acknowledgements
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

See related subject areas:

  • biophysics
  • biomimetics

Related articles

Cited by

Interface Focus To read similar articles, check out our sister journal

Open biology

  • Interface
    • About this journal
    • Contact information
    • Purchasing information
    • Submit
    • Author benefits
    • Open access membership
    • Recommend to your library
    • FAQ
    • Help

Royal society publishing

  • ROYAL SOCIETY PUBLISHING
    • Our journals
    • Open access
    • Publishing policies
    • Conferences
    • Podcasts
    • News
    • Blog
    • Manage your account
    • Terms & conditions
    • Cookies

The royal society

  • THE ROYAL SOCIETY
    • About us
    • Contact us
    • Fellows
    • Events
    • Grants, schemes & awards
    • Topics & policy
    • Collections
    • Venue hire
1742-5662

Copyright © 2018 The Royal Society